Composite

Part:BBa_K318502:Design

Designed by: Sarah R. Sandock   Group: iGEM10_Wisconsin-Madison   (2010-09-27)

lacI pL + RBS + RcsB + RBS + RcsA + TT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We wanted to test what IPTG induction levels were appropriate to protect our cells from an acidic environment of pH=2 before placing our devices under constitute control with appropriate RBS's.

Source

The RcsA gene we used was originally submitted by CalTech 2008 BBa_K137113 and the RcsB gene we used was originally submitted by Imperial 2009 BBa_K200000.

References